Region DNA Fragments
نویسندگان
چکیده
Koji Nomura ', Wataru Saito Kimiyo Ono2, Hiromitsu Moriyama3, Seiko Takahashi4, Inoue4 and Kiyoshi MasudaS i institute ofAgriculture and R)restt),, University of Tlsukubq 71vukuba, Ibaraki, 305 Jopan 2 institute ofBiologicat Science, Uhiversity of 7lsukuba, 7lsukuba, Ibaraki, 305 Japan 3 Ricuity ofAgricutture, Tbkyo Uhiversity ofAgrieutture and Tlechnology, Fuchu, Tbkyo, 183 Japan ` Division of Cell Biology, Akita Prqfecturat CoUege ofAgriculture, Ohgata, Akita, OIO-04 Jopan 5 Department of Hbrticulturat Science, thculty ofAgriculture, Hokkaido Uhiversity, Scmporo, 060 Jopan Masayasu
منابع مشابه
طراحی و ساخت DNA Ladder صد جفت بازی با روش تلفیقی PCR و هضم آنزیمی
Background: Molecular DNA markers are one of the most important tools in molecular biology labs. The size of DNA molecules is determined by comparing them with known bands of markers during gel electrophoresis. There are many different protocols to produce these kinds of molecular markers. In this study we have suggested an efficient strategy to produce molecular weight markers in industrial pr...
متن کاملDETECTION AND RESTRICTION ANALYSIS OF C YTOMEGALOVIRUS DNA PERSISTING IN HUMAN ATHEROSCLEROTIC PLAQUES USING POLYMERASE CHAIN REACTION
The polymerase chain reaction (PCR) as applied to detection of a foreign DNA in clinical specimens could provide a sensitive instrument for rapid detection of viral DNA persisting in tissues of patients suspected of latent infection. Human cytomegalovirus (HCMV) DNA was found in arterial plaques of patients with atherosclerotic lesions using a PCR assay with nested primer oligonucleotides ...
متن کاملCloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.
The organization of the kappa chain constant region gene was compared in DNA from an immunoglobulin-producing mouse myeloma (MOPC 173) and from liver. In situ hybridization using the Southern blotting technique revealed constant region gene-containing EcoRI-DNA fragments of 14 and 20 kb in the myeloma tissue whereas one EcoRI-DNA fragment with a length of 15 kb was found in liver DNA. After enr...
متن کاملPattern of DNA cytosine methylation in Aeluropus littoralis during temperature stress
DNA methylation as an epigenetic mediator plays the important role in spatial and temporal gene regulation and ensures the stability and the plasticity of organism. In this investigation, methylation sensitive amplification polymorphism (MSAP) were assessed in CCGG sites on a halophytic plant, Aeluropuslittoralis in response to different temperature stresses including freezing...
متن کاملAnalysis of SFL1 and SFL2 Promoter Region in Arabidipsis thaliana using Gateway Cloning System
SFL1 and SFl2 (SETH Four Like) genes are two members of SETH4 gene family in Arabidopsis thaliana expressed in saprophytic tissues. In this study, expression of SFL1 and SFL2 genes were studied using Gateway Cloning Technology. Primers were designed for PCR amplification of promoter region of SFL1 (900 bp) and SFL2 (930 bp) genes having attB1 recombination sites using Kod Hi Fi DNA polymerase e...
متن کاملOrganization of the Ribosomal RNA Gene Cluster in Lytechinus
The DNA coding for ribosomal RNA from Lytechinus variegatus, an Atlantic sea urchin species, is arranged in repeating units. Each unit is cleaved into four fragments by the restriction endonuclease Eco RI. The same gene region is dissected by the enzyme Sal 1 to yield two fragments. The restriction nuclease Kpn I has one restriction site within each repeat unit, thus generating a single fragmen...
متن کامل